Kev piav qhia
Cagrilintide hmoovyog ib tug ntev - ua tib neeg amylin analogue optimized los ntawm esterification thiab sequence engineering, teej tug mus rau amylin -calcitonin receptor tsev neeg ntawm agonists. Nws lub luag haujlwm tseem ceeb yog qhov kev noj tshuaj txhua lub lim tiam tswj hwm nws cov teeb liab ua haujlwm, ua rau nws tsim nyog rau kev tshawb fawb txog kev siv tshuab thiab tshuaj pharmacodynamic tsom mus rau txoj hauv kev satiety, kev tswj lub plab zom mov, thiab kev tswj hwm glucagon.
Lub Cagrilintide muab los ntawm Shaanxi Medibridge yog kev tshawb fawb -qib (RUO) lyophilized hmoov. Kev tswj kom zoo npog cov cim tseem ceeb xws li kev txheeb xyuas, purity, impurities thiab residues, ntsev daim ntawv, thiab cov ntsiab lus. Tsawg endotoxin / dedicated buffer systems thiab customized daim ntawv lo versions muaj kom tau raws li cov kev xav tau ntawm cellular thiab tsiaj pharmacodynamic thwmsim.
COA
|
Yam khoom |
Txuj |
Cov txiaj ntsig |
|
Purity |
Ntau dua lossis sib npaug li 98% |
99.85% |
|
Peptide Assay |
Ntau dua lossis sib npaug li 80% |
92.66% |
|
Loj Spectrum |
4409 |
4409 |
|
Solubility |
Soluble hauv dej |
Ua raws |
|
Clarity thiab xim ntawm kev daws |
Ntshiab thiab tsis muaj xim |
Ua raws |
|
Sodium ntsev |
<5.0% |
1.33% |
|
Dej |
Tsawg dua lossis sib npaug li 7.0% |
2.58% |
|
Residual Solvent: Methanol |
||
|
Tsawg dua lossis sib npaug li 0.3% |
0.0054% |
|
|
Isopropanol |
Tsawg dua los yog sib npaug li 0.5% |
N.D. |
|
Cov tshuaj acetonitrile |
Tsawg dua lossis sib npaug li 0.041% |
0.039% |
|
Methylene Chloride |
Tsawg dua lossis sib npaug li 0.06% |
0.019% |
|
N,N{0}}Dimethylformamide |
Tsawg dua lossis sib npaug li 0.088% |
0.051% |
|
Triethylamine |
Tsawg dua lossis sib npaug li 0.032% |
N.D. |
|
Tert -butyl methyl ether |
Tsawg dua los yog sib npaug li 0.5% |
0.232% |
|
Endotoxin |
Tsawg dua lossis sib npaug li 0.5 EU / mg |
Ua raws |
|
Microbial txwv |
Tag nrho cov kab mob aerobic<100 CFU/g Tag nrho cov poov xab & pwm<50 CFU/g |
<50 CFU/g <10 CFU/g |
|
Cia |
Khaws rau hauv qhov chaw tsaus thiab txias qhuav (-20 txog 8 degree) |
|
|
Xaus |
Cov batch ua raws li IN-HOUSE tus qauv |
|
Specifications (rau kev tshawb fawb siv nkaus xwb)
|
Daim ntawv |
Qauv Order |
Specification |
|
Cov hmoov nyoos |
1 g |
Purity yog NLT 99.85% |
|
Vials |
10 vials |
3ml / 5ml / 7ml / 15ml vials thiab lwm yam. |
Kev Qhia Txog Kev Tshawb Fawb (Rau kev sib pauv kev kawm nkaus xwb, tsis muaj kev siv tshuaj kho mob.)
Kev Tshawb Fawb Cov Lus Qhia Zoo rau Cagrilintide
Kev tshawb fawb tsom mus rau qhov sib txawv ntawm qhov sib txawv ntawm CTR + RAMP1/2/3 complex. cAMP, -arrestin, thiab ERK lub sij hawm nkhaus tuaj yeem ua tau zoo hauv kev qhia HEK293/CHO cell kab los piv Emax/EC50 thiab kinetic (on/off-rate) qhov sib txawv, ntsuam xyuas qhov muaj kev tsis ncaj ncees agonism.
Kev tsom mus rau kev sib txuas ntawm ligand binding thiab receptor endocytosis: BRET/NanoBiT lossis TR-FRET binding kinetics ua ke nrog confocal internalization trajectories tuaj yeem pab piav qhia cov txiaj ntsig persistence thiab desensitization.
Cov ntsiab lus tseem ceeb: Tsawg- adsorption consumables; 0.1% BSA lossis sib npaug cov neeg nqa khoom kom txo tau peptide adsorption; aliquoting thiab cryopreservation kom tsis txhob khov -thaw cycles; pab pawg tswj yuav tsum suav nrog ob tsev neeg peptides thiab inactivated mutant peptides los pab txhawb kev sib txawv ntawm receptor- cov teebmeem tshwj xeeb.
GI Motility & Postprandial Hormones
Hauv cov qauv tsiaj, kev ua pa hauv plab tuaj yeem ntsuas tau siv acetaminophen (APAP) txoj kev. Ua ke nrog postprandial ntshav qabzib, glucagon, thiab insulin nkhaus, cov ntaub ntawv pov thawj ntawm "kev qeeb plab hnyuv- txo qis ntshav qabzib hloov pauv" tuaj yeem tsim.
Hauv vitro, qhov cuam tshuam tsis ncaj ntawm txoj hnyuv (GLP-}1, PYY) tso tawm tuaj yeem pom tau siv cov kab mob hauv plab lossis cov kab mob organoids los txheeb xyuas qhov muaj nyob ntawm plab hnyuv tawm tswv yim.
Nco ntsoov tias muaj zog alkaline / siab organic solvent systems tuaj yeem cuam tshuam rau peptide stability; kinetic thwmsim tau pom zoo kom ua nyob rau hauv ib tug tsis system nrog ib tug pH ntawm 4.5-6.5.
Obesity qauv & Pharmacodynamics
DIO nas / nas yog cov qauv yooj yim:Cagrilintide hmoovKev tswj hwm tau rov ua dua rau ob peb lub lis piam, saib xyuas lub cev hnyav, lub cev rog (EchoMRI / DXA), thiab cov qauv noj mov, thiab kev siv hluav taws xob thiab kev ua pa (RER) raug soj ntsuam siv cov kab mob metabolic.
Kev siv cov kev tswj kev noj zaub mov ua ke tuaj yeem cais cov txiaj ntsig ntawm "kev noj zaub mov tsis txaus" thiab "kev siv zog ntau ntxiv"; Kev sib xyaw ua ke no nrog OGTT/ITT lossis hyperinsulin{0}}euglycemic clamps txhim kho cov lus pom zoo ntawm cov piam thaj metabolism hauv cov lus xaus.
Nws raug nquahu kom ntxiv cov ntsuas kev coj tus cwj pwm (xws li nas lithotripsy / kaolin ingestion thiab conditioned saj aversion) kom sib txawv ntawm pharmacological anorexia thiab kev phiv tshuaj.
Kev sib xyaw ua ke & Synergy
Kev sib xyaw ua ke nrog GLP-1/GIP/GLP-1+GIP peptides, SGLT2 inhibitors, lossis glucagon receptor modulators tuaj yeem tsim cov teebmeem sib txawv ntawm qhov sib txawv, suav nrog kev tswj hwm kev noj zaub mov, ntshav qabzib tom qab, thiab lipid metabolism.
Tsim cov koob tshuaj matrices thiab sib npaug sib koom ua ke (Bliss / Loewe / HSA) los ntsuas cov khoom sib xyaw ua ke; tswj titration tus nqi thiab sau cov pib thiab nyem lub sij hawm ntawm plab hnyuv tsis xis nyob kom sib npaug kev ua tau zoo thiab kam rau ua.
Cov ntsiab lus tseem ceeb analytical: Seb lub toj siab theem qeeb, txawm tias rov qab los yog txo qis, thiab kev kho qhov hnyav tom qab txiav cov tshuaj.
Hepatic lipid metabolism & NAFLD / NASH
Hauv WD + fructose lossis MCD qauv, peb pom cov kab mob siab lipid deposition thiab inflammatory fibrosis markers (siab TG, H&E / Oil Red O, NAS score, Sirius Red), ua ke nrog transcriptomics / lipomics tsom xam kom paub meej txog txoj kev hloov.
Tib lub sijhawm, peb tau soj ntsuam cov ntshav lipids (TG, VLDL, tsis yog - esterified fatty acids) thiab cov cim inflammatory (CRP/cytokines) los tsim cov kab mob ntawm "kev poob phaus- lipid metabolism remodeling- nce o."
Nrog rau cov tshuaj insulin hauv siab (glucose production inhibition) yog qhov kawg pab kom paub qhov txawv ntawm qhov yooj yim yuag poob thiab lub siab- cov teebmeem tshwj xeeb.
FAQ
Q: Dab tsi yog qee cov txheej txheem kev nyab xeeb yog tias kev tshem tawm tsis ua tiav?
A: Cia cov hmoov lyophilized sov rau chav sov li 5-10 feeb, tsis txhob sib cuag ncaj qha ntawm condensate thiab hmoov.
Dissolve nrog me me ntawm acidified dej (piv txwv li, 2 mM HCl los yog 0.1% glacial acetic acid, lub ntim yuav tsum txaus kom ntub cov hmoov).
Q: Cov kuab tshuaj pom zoo thiab pH ntau npaum li cas?
A: Nws raug nquahu kom siv sijhawm ntev - khaws cia thiab siv nyob rau hauv qhov tsis sib xws nrog pH ntawm 4.5–6.5 (xws li acetate / citrate system). Tsis txhob muaj zog alkaline ib puag ncig (pH> 8), muaj zog oxidizing cov neeg ua haujlwm, thiab ib puag ncig uas muaj feem ntau ntawm cov kuab tshuaj organic.
Q: Cia thiab aliquoting cov lus pom zoo tom qab reconstitution?
A: Cagrilintide hmoov: Khaws rau hauv qhov chaw txias, qhuav kom deb ntawm lub teeb ntawm -20 txog -80 degree; batch stability feem ntau yog 18-24 lub hlis (raws li COA). Txo cov voj voog khov-thaw (tsis pub ntau tshaj 2 zaug pom zoo). Tam sim ntawd tom qab thawj reconstitution, aliquot cov hmoov rau hauv vaj huam sib luag rau kev ntsuam xyuas.
Q: Kev thauj mus los, kev txais, thiab kev pom zoo?
A: me ntsis shrinkage lossis adhesion ntawm khov - cov blocks qhuav yog qhov qub thiab tsis cuam tshuam qhov zoo. Thov hu rau peb tam sim yog tias koj pom qhov txawv txav deliquescence lossis ntim puas.
Q: Qhov no puas tuaj yeem siv ncaj qha rau tsiaj / chaw kho mob?
A: Cov khoom no yog siv rau kev tshawb fawb nkaus xwb (RUO) thiab yuav tsum tsis txhob siv rau tib neeg lossis kws kho tsiaj kuaj mob, kev kho mob, lossis kev tswj xyuas ncaj qha.
Q: Koj puas muaj kev tsim khoom thiab kev tswj xyuas zoo?
A: Peb muaj tag nrho peptide R & D thiab ntau lawm platform. Peb cov txheej txheem zoo yog tswj hwm thiab sau tseg raws li GMP cov qauv. Peb tuaj yeem muab tag nrho COA / SDS thiab batch traceability; peb kuj txhawb cov kev pabcuam customized xws li kev sau ntawv thiab qib endotoxin tsawg.
Kev siv Cagrilintide rau kev kho mob rog yog ib txoj hauv kev tshiab thiab pheej hmoo rau kev txhim kho glycemic tswj. Yog tias koj xav tau tus khub peptide siab dawb rau kev koom tes ua haujlwm ntev, email hi@medibridgeapi.com. Lossis xa lus rau peb ntawm WhatsApp ntawm +44 07548632075.
Cim npe nrov: Tuam Tshoj cagrilintide hmoov manufacturers, lwm tus neeg, Hoobkas
